This ssystem was developed for de-hydration and infiltrating samples with plastics (for example, Technovit 7200), for application in medical and biomaterial research.

With increasing development of cut, thin-ground technology, the problems of preparing bones that are not decalcified with and without implants also increased. Open questions such as remaining water in the tissue and insufficient infiltration drove the device development to advance. 

The E 510 is tailored to users with small numbers of samples but high quality demands. Essential for the diffusion procedures is the change of the media concentration on the tissue surface. This means that the liquid leaving the tissue does not remain on the surface, but it is rinsed away through permanent agitation. 

For a de-hydration and infiltration program of 12 days (standard), with its six light-proof containers, the E 510 offers the opportunity to take another step with increasing alcohol concentration every two days. In routine operation, after 12 days a container with the finished prepared samples can be removed for the first time, and every two days, new samples are available.

Samples with bone cement on GMA basis can be de-hydrated under vacuum.

Features:

• 6 aluminum containers with light-proof seals (for light-sensitive embedding plastic such as Technovit 7200), which can be shaken at the same time
• 10 sample glasses with ground-in covers
• 10 stainless steel containers for samples
• Vacuum de-watering possible at 7-10 mbar in 2 containers for samples with bone cement (GMA de-hydration)
• Variable speed regulation
• Double head vacuum pump, optional

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The EXAKT 520 light polymerization device is used to embed medical and industrial samples in plastics.

Features:

• Minimal thermal effect on the sample
• Stable polymerization with a high degree of hardening
• Minimal bubble and crack formation
• Reusable embedding trays
• Opportunity for water cooling

Technical specifications:

• Weight 12.5 kg
• Workspace L = 600 mm x D = 450 mm x 400 mm
• Voltage 220/110 V
• Output 30 W
• When using cooling, a water feed and drain is required
• Water requirement 3-4 l/h

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After cutting open a polymerized block, sometimes tissue zones appear that are not sufficiently infiltrated and polymerized. The most frequent cause of this is insufficient de-hydration. If tissue zones are still soft, the sample is difficult to cut and grind, it clogs up the tools. If, however, the sample is processed further regardless, there is a danger that during the grinding and polishing, water can get into the flaws and detach the sample from the object carrier in the last phase of the thin-grinding procedure.

With the EXAKT re-infiltrator, the poorly-infiltrated preparation can be dried, re-infiltrated with liquid plastic (Technovit 7200), and then polymerized sufficiently.

For users who work with bones or with thick samples that are embedded in plastic, the selection of our re-infiltration process is the variant that saves time and money.

Features:

• Warming plate, temperature value constant 40°C
• Polymerization with blue light
• Time control of the polymerization
• Vacum cover, ø 150 mm
• Vacuum pump for drying and polymerization, 35mbar abs.

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For histological samples and for other applications that require thin grinding (10 - 25 µm), it is absolutely necessary to work plane-parallel during the entire sample processing. The EXAKT 401 vacuum adhesive press was developed in order to apply plane-parallel samples to slides without having to grind the surface in advance. The vacuum adhesive press works, for example, in combination with Technovit 4000 adhesive.

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If you want to process a tissue with an implant into a thin-ground sample, the key to success is the perfect placement of the sample on the grinding medium. For transmission microscopy, the transparency of the adhesive layer in combination with the object carrier is just as important.

For the further optimization of the cutting/ thin-ground process, an adhesive (Technovit 7210) was developed in cooperation with Heraeus Kulzer. This adhesive is clear, it enables a parallel adhesive coating, and it hardens only with the application of blue light.

The transparent support bracket of the vacuum adhesive press enables the researcher and the technician to observe the distribution of the adhesive and detect possible air incursions immediately. The necessary corrections can be made easily before the polymerization.

The use of the precision adhesive press enables the developer and the technician to create perfect, even thickness adhesive layers.

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